Regulatory effects of nerve growth factor on the differentiation of goat bone marrow mesenchymal stem cells into osteoblasts

Objective: To investigate the effects of nerve growth factor(NGF) on the differentiation of goat bone marrow mesenchymal stem cells(BMSCs) into osteoblasts.Methods: The goat BMSCs were cultured in vitro and the marker proteins on the BMSCs surface were identified by flow cytometry.The third passage of BMSCs were randomly divided into blank control group,osteoblasts control group,NGF group and experimental group.The activities of alkaline phosphatase(ALP) and osteocalcin(0C) were examined and the of von Kossa staining method was used to observe the osteogenic differentiation.Results: CD90 and CD105 were strong positive while the CD34 and CD45 were negatively expressed in BMSCs.The activities of ALP and OC was significantly higher in the experimental group than that in the other three groups(P0.05).The staining of von Kossa was positive and the black calcium nodules were appeared in the the osteoblasts control group.The number and the area of the calcium nodules were greater in the experimental group.But there were no significant differences of each index between the NGF group and the blank control group.Conclusion: NGF can′t induce goats BMSCs to osteoblasts,but can clearly promote the differentiation of goats BMSCs.