Study on preparation of antioxidant peptides of silver carp proteins

In order to prepare the silver carp protein peptides which have antioxidant activity,silver carp protein was hydrolysed with 5 common proteases in the same enzyme activity.The hydrolysis degree and DPPH radical-scavenging activity of hydrolysate were determined by TNBS method and spectrophotometer.The relativity between the degree of hydrolysis and antioxidant activity were also analyzed.The results showed that the highest hydrolysis degree of hydrolysate was hydrolysed 5 h by Alcalase 2.4L(P﹤0.05).Pepsin hydrolysate was the most powerful on scavenging DPPH radical and reducing activity and its eliminating rate was 61% and 1.6,respectively.There were marked relativity between the degree of hydrolysis and metal-chelating activity,and also between reducing power and metal-chelating activity(P﹤0.05).Different proteases had different effects on hydrolysis degree and antioxidants activity.