Preparation of Antioxidant Peptide from Common Carp(Cyprinus Carpio) Myofibrillar Protein by Enzymatic Hydrolysis

The enzymolysis effects of papain,alcalase and trypsin was studied respectively on the common carp(Cyprinus carpio) myofibrillar protein,taking the degree of hydrolysis(DH) and the the scavenging rate of DPPH free radical as index.The results of experiment indicated that papain was the optimum hydrolase for preparing enzymatic hydrolysate of common carp myofibrillar protein.The optimal conditions of hydrolysis was: temperature 55 ℃,pH7,adding enzyme 1 000 U/g,concentration of substrate 4 %,and hydrolysis 2 h by single factor test and the orthogonal experiment.In this condition,the scavenging rate of DPPH free radical were 82.73 %,enzymatic hydrolysate of common carp myofibrillar protein showed high antioxidant activity.