2007•Zhongguo fuyou baojianRequires access
Study on evaluating acrosome reaction function by acroin activity released acrosome reaction
Hong Wang
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Abstract
Objective:To investigate acrosome reaction function by acroin activity released acrosome reaction.Methods:Human semen was obtained from 10 fertile donors with normal semen parameters. Specimens were allowed to liquefy at 37℃,and were prepared by Percoll-gradient treatment. Spermatozoa were recovered from the upper layer. Acrosome reaction was analyzed using FITC-PSA staining. Acrosin activity was analyzed using the colorimetric by spectrocolorimeter.Results:When calcium ionosphere A23187 and progesterone induced acrosome reaction,the percentage of acrosome reacted sperm and released acrosin activity were both markedly increased(P0.05).With increase of final concentration of A23187,the percentage of acrosome reacted sperm and released acrosin activity were both markedly increased(P0.01). There was a markedly positive relative between the percentage of acrosome reacted sperm and released acrosin activity(r=0.950,P0.001).With increase of final concentration of progesterone,the percentage of acrosome reacted sperm and released acrosin activity were both markedly increased(P0.05).There was a markedly positive relative between the percentage of acrosome reacted sperm and released acrosin activity(r=0.921,P0.001).Conclusion:When progesterone induced acrosome reaction,released acrosin activity can evaluate this sperm function.