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2003•Guizhou nongye kexueRequires access

Propagation of Male-sterile Radish by Tissue Culture

Ren Tong-hui

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Abstract

Studies on micropropagation of radish were carried out on CMS lines A1. The results showed that high frequency of adventitious shoot could be gotten by using the tip of stem with cotyledon as explants. For adventitious shoot genesis, the optimum medium was base medium with 6-BA(4mg/L) + NAA (0. 4mg/L). Medium supplemented 6-BA 4mg/L + NAA 0. 2mg/L was optimum for the number of regenerated shoots per explant. Enhancing the rate of 6-BA/NAA was essential for keeping high frequency of adventitious shoot after several times of subculture.

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What this paper is about

Studies on micropropagation of radish were carried out on CMS lines A1. The results showed that high frequency of adventitious shoot could be gotten by using the tip of stem with cotyledon as explants. For adventitious shoot genesis, the optimum medium was base medium with 6-BA(4mg/L) + NAA (0. 4mg/L). Medium supplemented 6-BA 4mg/L + NAA 0. 2mg/L was optimum for the number of regenerated shoots per explant. Enhancing the rate of 6-BA/NAA was essential for keeping high frequency of adventitious shoot after several times of subculture.

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Available abstract

Studies on micropropagation of radish were carried out on CMS lines A1. The results showed that high frequency of adventitious shoot could be gotten by using the tip of stem with cotyledon as explants. For adventitious shoot genesis, the optimum medium was base medium with 6-BA(4mg/L) + NAA (0. 4mg/L). Medium supplemented 6-BA 4mg/L + NAA 0. 2mg/L was optimum for the number of regenerated shoots per explant. Enhancing the rate of 6-BA/NAA was essential for keeping high frequency of adventitious shoot after several times of subculture.

Key concepts: Subculture (biology), Explant culture, Shoot, Micropropagation, Cotyledon, Tissue culture, Basal shoot, Botany

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