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STUDY ON THE PREPARATION TECHNIQUE OF COLLAGEN FROM SQUID(OMMASTREPHES BARTRAMI) SKIN

Zeng Qing-zhu

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Abstract

To explore the components of squid skin and extraction technique of collagen from squid skin.Preparation methods of collagen from pretreated squid skin by hot water, acid and enzymatic hydrolysis were investigated, respectively.The results showed that squid skin had abundant protein, the content of crude protein in squid was 75.35 % (dry weight basis) and the content of collagen in squid skin was 53.96 % (dry weight basis), collagen content in crude protein was 71.61 %. The extraction yield of collagen was 90.26 % by hot water and 95.16 % by acid. The highest extraction yield of collagen was 97.56 % by papain enzymatic hydrolysis. The best condition of enzymatic hydrolysis was that E:S 1∶20, temperature50 ℃,pH6.0,quantity of enzyme used was 3 200 U/g and time was 6 h by papain hydrolysis. The best preparation method of collagen from squid skin which had higher extraction yield was enzymatic hydrolysis with papain.

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What this paper is about

To explore the components of squid skin and extraction technique of collagen from squid skin.Preparation methods of collagen from pretreated squid skin by hot water, acid and enzymatic hydrolysis were investigated, respectively.The results showed that squid skin had abundant protein, the content of crude protein in squid was 75.35 % (dry weight basis) and the content of collagen in squid skin was 53.96 % (dry weight basis), collagen content in crude protein was 71.61 %. The extraction yield of collagen was 90.26 % by hot water and 95.16 % by acid. The highest extraction yield of collagen was 97.56 % by papain enzymatic hydrolysis. The best condition of enzymatic hydrolysis was that E:S 1∶20, temperature50 ℃,pH6.0,quantity of enzyme used was 3 200 U/g and time was 6 h by papain hydrolysis. The best preparation method of collagen from squid skin which had higher extraction yield was enzymatic hydrolysis with papain.

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Available abstract

To explore the components of squid skin and extraction technique of collagen from squid skin.Preparation methods of collagen from pretreated squid skin by hot water, acid and enzymatic hydrolysis were investigated, respectively.The results showed that squid skin had abundant protein, the content of crude protein in squid was 75.35 % (dry weight basis) and the content of collagen in squid skin was 53.96 % (dry weight basis), collagen content in crude protein was 71.61 %. The extraction yield of collagen was 90.26 % by hot water and 95.16 % by acid. The highest extraction yield of collagen was 97.56 % by papain enzymatic hydrolysis. The best condition of enzymatic hydrolysis was that E:S 1∶20, temperature50 ℃,pH6.0,quantity of enzyme used was 3 200 U/g and time was 6 h by papain hydrolysis. The best preparation method of collagen from squid skin which had higher extraction yield was enzymatic hydrolysis with papain.

Key concepts: Papain, Squid, Extraction (chemistry), Hydrolysis, Enzymatic hydrolysis, Chemistry, Chromatography, Yield (engineering)

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STUDY ON THE PREPARATION TECHNIQUE OF COLLAGEN FROM SQUID(OMMASTREPHES BARTRAMI) SKIN — Research Paper | ScholarLens