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2006•Unpublished venueRequires access

Experimental study of angiogenin factors secreted by bone marrow mononuclear cells cultured in vitro

X Zhang

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Abstract

Objective To define the secretory capability of angiogenin factors by bone marrow mononuclear cells(BM-MNC) cultured in vitro.Methods Rat BM-MNCs were isolated by centrifugation through a Histopaque density gradient and cultured for four weeks in vitro.The levels of vascular endothelial cell growth factor(VEGF),basic fibroblast growth factor(bFGF) and interleukin-1β(IL-1β) were tested by enzyme-linked immunosorbent assay(ELISA).Results The levels of VEGF in the conditioned medium at 1,2,3 and 4 weeks were(24.40±7.99),(89.28±5.13),(115.24±10.08) and(157.00±15.64) pg/ml,respectively.The levels of bFGF at 1,2,3 and 4 weeks were(52.72±2.13),(48.10±6.41),(44.71±3.21) and(25.61±2.42) pg/ml respectively,and those of IL-1β were(31.28±5.44),(71.87±3.01),(55.77±11.94) and(41.75±9.14),respectively.Conclusion The BM-MNCs can persistently secrete potent angiogenic cytokines such as VEGF,bFGF and IL-1β.

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Objective To define the secretory capability of angiogenin factors by bone marrow mononuclear cells(BM-MNC) cultured in vitro.Methods Rat BM-MNCs were isolated by centrifugation through a Histopaque density gradient and cultured for four weeks in vitro.The levels of vascular endothelial cell growth factor(VEGF),basic fibroblast growth factor(bFGF) and interleukin-1β(IL-1β) were tested by enzyme-linked immunosorbent assay(ELISA).Results The levels of VEGF in the conditioned medium at 1,2,3 and 4 weeks were(24.40±7.99),(89.28±5.13),(115.24±10.08) and(157.00±15.64) pg/ml,respectively.The levels of bFGF at 1,2,3 and 4 weeks were(52.72±2.13),(48.10±6.41),(44.71±3.21) and(25.61±2.42) pg/ml respectively,and those of IL-1β were(31.28±5.44),(71.87±3.01),(55.77±11.94) and(41.75±9.14),respectively.Conclusion The BM-MNCs can persistently secrete potent angiogenic cytokines such as VEGF,bFGF and IL-1β.

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Available abstract

Objective To define the secretory capability of angiogenin factors by bone marrow mononuclear cells(BM-MNC) cultured in vitro.Methods Rat BM-MNCs were isolated by centrifugation through a Histopaque density gradient and cultured for four weeks in vitro.The levels of vascular endothelial cell growth factor(VEGF),basic fibroblast growth factor(bFGF) and interleukin-1β(IL-1β) were tested by enzyme-linked immunosorbent assay(ELISA).Results The levels of VEGF in the conditioned medium at 1,2,3 and 4 weeks were(24.40±7.99),(89.28±5.13),(115.24±10.08) and(157.00±15.64) pg/ml,respectively.The levels of bFGF at 1,2,3 and 4 weeks were(52.72±2.13),(48.10±6.41),(44.71±3.21) and(25.61±2.42) pg/ml respectively,and those of IL-1β were(31.28±5.44),(71.87±3.01),(55.77±11.94) and(41.75±9.14),respectively.Conclusion The BM-MNCs can persistently secrete potent angiogenic cytokines such as VEGF,bFGF and IL-1β.

Key concepts: Angiogenin, In vitro, Basic fibroblast growth factor, Peripheral blood mononuclear cell, Bone marrow, Vascular endothelial growth factor, Differential centrifugation, Chemistry

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