Inhibitory effect of recombinant IFN-α combined with endostatin gene on corneal neovascurization induced by alkali burn

Objective To investigate the inhibitory effect of recombinant IFN-α combined with endostatin gene on corneal neovascurization induced by alkali burn.Methods Lipofectin with GFP expression vector was injected into subconjunctival tissue in eye of rabbit.Three days later,the expression of GFP in cornea was observed with a laser confocal microscope.CNV animal model was developed by alkali burn,recombinant IFN-α and lipofectin with endostatin expression vector were injected into the subconjunctival tissue in eye of rabbit.The inhibitory effect of them on corneal neovascurization induced by alkali burn was observed with slitlamp microscopy.Results There was expression of GFP in the cornea of experimental group,but not in the cornea of control group.Combining recombinant IFN-α with endostatin gene to treat corneal neovascurization,the results showed the lengths and areas of corneal neovascurization on the 7th,10thand 13rd day in combined treatment group were significantly less than that in recombinant IFN-α treatment group and endostatin gene treatment group(P0.05).Conclusion Recombinant IFN-α combined with endostatin gene can effectively inhibit corneal neovascurization induced by alkali burn.