Effect of Snail on the invasion and metastasis of cervical cancer cells

Objective To study the relationship of Snail with invasion and metastasis of cervical cancer,and to explore its molecule mechanism.Methods Snail over-expression plasmid was transfected into cervical cancer cell lines of HeLa and SiHa.Western blot was applied to analyze the corresponding molecules including Snail,E-cadherin and Vimentin.The invasion and metastatic ability was examined by in vitro cell wound healing experiment and Boyden chamber invasion assay.Results ①After transfection of pEGFPC1/Snail into cervical cancer cell lines of HeLa and SiHa,the expression of E-cadherin was significantly lower than its counterpart,however,the expression of Snail and Vimentin was significantly higher than its control group.②The capability of wound closure in SiHa and HeLa cells transfected with pEGFPC1/Snail at 12 h was significantly enhanced(P0.05),and the invading/migrating cells were obviously increased at 12 h after transfection of pEGFPC1/Snail into SiHa and HeLa cells(P0.05).Up-regulation of Snail could enhance the capability of metastasis and invasion.Conclusion Transcription factor of Snail could enhance the potential of invasion and metastasis of cervical cancer cell lines through induction of Epithelial-mesenchymal transition(EMT).