Overexpression of Snail Enhances the Invasive Capacity of Human Lung Cancer Cell A549 in Culture

The invasion change of human lung cancer cell A549 was detected with overexpression of snail transcription factor inducing epithelial mesenchymal transition (EMT) to supply evidence for selection of new targeting molecular drugs.pcDNA3.1-snail vector was constructed and transfected to number of A549 cell,then cells being selected with G418 to construct the EMT cell model of lung cancer cell line.The morphological change and the expression change of E-cadherin, vimentin were observed by immunocytochemistry,immunofluorescence and Western blotting.Invasive capacity of A549 cell was detected by transwell invasion chamber in vitro.After being transfected with pcDNA3.1-snail,the A549 cells become long and thin,sparse,the degree of cell fusion decreased.The results indicated that the expression of E-cadherin was down regulated and the expression of vimentin was up regulated.Results of transwell assay showed that number of A549 cells penetrated to the membrane were obviously increased.In short,snail can induce non-small lung cancer to EMT and enhance non-small lung cancer in vitro invasion capability.