STUDYING THE EFFECT OF SODIUM FLUORIDE ON OSTEOCLAST IN VITRO

Objective:To study the influence of sodium fluoride(NaF)on osteoclast in vitro.Methods:Osteoclast isolated from the long bone of neonatal rabbits were cultured on glass and bone slices.After cultured 6 hours,different concentrations(10 -7 mol/L～10 -3 mol/L)of NaF were administered.The effect of NaF on osteoclast was examined by the method of enzyme histochemistry staining.The number and area of lacuna formed by osteoclast resorption on bone slices were observed and analyzed with phase-contract microscope and image analysis apparatus.Results:NaF (10 -5 mol/L,10 -4 mol/L,10 -3 mol/L) made osteoclast which was identified as tartrate resistant acid phosphatase (TRAP) staining positive multinucleated cell on glass slices count reduced,volume decreased and vacuolar developed,especially at the concentration of 10 -3 mol/L( P 0 05).In addition,compared with the control group,the number and area of bone resorption lacuna on bone slices reduced in the group given 10 -5 mol/L,10 -4 mol/L,10 -3 mol/L NaF ( P 0 05).Furthermore,the border of resorption lacuna became clouding.Conclusion:Within experimental concentrations and time,NaF could directly destroy osteoclast and inhibit bone resorption function of osteoclast in vitro.