2007•Lishizhen Medicine and Materia Medica ResearchRequires access

Analysis of Monosaccharide Compositions in Epimedium Polysaccharide by Precolumn Derivation HPLC

Jiayu Jiang

Abstract

Objective To determine the monosaccharides in the Epimedium polysaccharide(EPS) and their molar ratio.Methods The Epimedium polysaccharide were hydrolyzed into monosaccharides with 1 mol/L sulfuric acid.The monosaccharide derivatives obtained with 1-Phenyl-3-methyl-5-pyrazolone(PMP) were separated by reverse-phase HPLC using a developed gradient elution process,and monitored by ultraviolet detector at 250 nm.Results Six monosaccharides derivatives were isolated with high sensitivity,good resolution by HPLC.The Epimedium polysaccharide is composed of mannose,rhamnose,galacturonic acid,glucose,galactose,and arabonose,with a molar ratio of 0.60∶0.74 ∶1.00 ∶0.29 ∶2.29 ∶1.43.Conclusion This method has accurate result and good reproducibility and can be used to determine the monosaccharides in Epimedium polysaccharide.

About this research paper

What this paper is about

Why it matters

OpenAlex reports 2 citations for this work. Citation counts describe recorded attention and do not establish research quality.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

Key concepts: Monosaccharide, Chromatography, Polysaccharide, Rhamnose, High-performance liquid chromatography, Chemistry, Epimedium, Galactose