EFFECT OF GLYCOSIDE HYDROLASE ON BIOAVAILABILITY OF HESPERIDIN AND NARINGIN

Objective To study whether the effect of glycoside hydrolase on bioavailability of hesperidin and naringin was through their sugar moieties.Method Male rats were administered i.g once with natural hesperidin 81.9 μmol(groupⅠ) and naringin 86.2 μmol(groupⅣ),hesperidinase treated hesperidin 81.9 μmol(groupⅡ)and naringinase treated naringin 86.2 μmol(groupⅤ) as well as hesperetin 81.6 μmol(groupⅢ) and naringenin 85.8 μmol(groupⅥ) respectively.Afterwards hesperetin and naringenin hydrolyzed with β-glucuronidase in plasma were detected.Results The peak plasma concentrations(Cmax) of hesperetin in Group Ⅱ were 70% and 188% higher than that in GroupⅠand GroupⅢ respectively.The time to reach the maxium concentration(Tmax) of plasma hesperetin in Group Ⅱ was 4h faster and 4h slower than that in GroupⅠ and Group Ⅲ respectively.The area under the curve(AUC) of plasma hesperetin in Group Ⅱ was 14% and 55.4% more than that in GroupⅠ and Group Ⅲ respectively.Similarly,Cmax of plasma naringenin in Group Ⅴ was one-fold and 25.7% higher than that in Group Ⅳ,and Group Ⅵrespectively.Tmax of plasma naringenin in GroupⅤ was 5h and 1.67 h faster than that in Group Ⅳ and GroupⅥ respectively,The AUC of plasma naringenin in Group Ⅵ was slightly higher than GroupⅤ,while that in Group Ⅴ was 34.5% higher than Group Ⅳ.Conclusion The bioavailability of hesperidin and naringin was improved by enzymatic treatment,thus absorbed easily.