Recent Developments of Protein Refolding by Size Exclusion Chromatography

High level expression of recombinant proteins in Escherichia coli often results in the formation of insoluble and inactive inclusion bodies,protein refolding from inclusion bodies is a puzzle in the production of recombinant proteins. Protein refolding by size exclusion chromatography (SEC) is a new and universal technology,which can refold many kinds of proteins of higher initial protein concentration,and has high bioactivity recovery compared with the common dilution method. The recent developments of protein refolding by SEC were reviewed.The mechanism of protein refolding by SEC,the factors affecting protein refolding,the future of this method were analyzed.