The expression of c-fos and c-jun protein in retina after retina ischemia reperfusion

Objective To determine in rats whether there is a change in c-fos and c-jun immediate early gene and interleukin-1β expression in retina after retinal ischemia reperfusion.Methods Retinal ischemia was induced in SD rat by means of raising the intraocular pressure to 130 mmHg(17.3 kPa) for 60-minute. Via cannulation into the anterior chamber, the model of retina ischemia reperfusion was established once releasing the pressure in the rat. The expression of c-fos, c-jun and IL-1β in paraffin section of retina was observed at 1 h, 3 h, 6 h, 12 h, 24 h after 60-minute ischemia by immunohistochemistry method.Results The expression of c-fos and c-jun first appeared in ganglion cell layer and inner nuclear layer in 1 h group, but not in outer nuclear layer. In 3 h group the expression of c-fos and c-jun arrived to the peak, and then decreased gradually in 6 h group. In 12 h and 24 h group, the expression of c-fos and c-jun arrived to normal level. But IL-1β appeared in ganglion cell layer and inner nuclear layer in 12 h and 24 h group. Weak signals for c-fos, c-jun and IL-1β were observed in the normal group.Conclusion It suggests that changes in expression of c-fos, c-jun and IL-β after retinal ischemia reperfusion,the phenomenon apoptosis is obvious in ganglion cell layer and inner nuclear layer after retinal ischemia reperfusion injury.