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2009•Journal of Clinical StomatologyRequires access

Effect of estrogen receptor beta on the expression of osteoprotegerin in human periodontal ligament cells

Ding Yi

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Abstract

Objective:To investigate the effect of ERβ on the expression of OPG in human periodontal ligament (HPLF)cells. Method:We employed a short interfering RNA (siRNA) technique to inhibit ERβ expression in HPLF cells and cells were cultured with saturating concentration of 17β-estradiol (10-7 mol/L) for 48 hours. Changes of the expression of OPG were determined by RT-PCR and Western blot analysis. Result:It was shown that estradiol caused an increase in OPG expression in HPLF cells. However,estradiol had no effect on the expression of OPG in HPLFsiERβ cells. Conclusion:These results indicate that ERβ is of major importance for estrogen-mediated effects on gene transcription in HPLF cells and estrogen influences the expression of OPG in HPLF cells via ERβ.

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Objective:To investigate the effect of ERβ on the expression of OPG in human periodontal ligament (HPLF)cells. Method:We employed a short interfering RNA (siRNA) technique to inhibit ERβ expression in HPLF cells and cells were cultured with saturating concentration of 17β-estradiol (10-7 mol/L) for 48 hours. Changes of the expression of OPG were determined by RT-PCR and Western blot analysis. Result:It was shown that estradiol caused an increase in OPG expression in HPLF cells. However,estradiol had no effect on the expression of OPG in HPLFsiERβ cells. Conclusion:These results indicate that ERβ is of major importance for estrogen-mediated effects on gene transcription in HPLF cells and estrogen influences the expression of OPG in HPLF cells via ERβ.

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Available abstract

Objective:To investigate the effect of ERβ on the expression of OPG in human periodontal ligament (HPLF)cells. Method:We employed a short interfering RNA (siRNA) technique to inhibit ERβ expression in HPLF cells and cells were cultured with saturating concentration of 17β-estradiol (10-7 mol/L) for 48 hours. Changes of the expression of OPG were determined by RT-PCR and Western blot analysis. Result:It was shown that estradiol caused an increase in OPG expression in HPLF cells. However,estradiol had no effect on the expression of OPG in HPLFsiERβ cells. Conclusion:These results indicate that ERβ is of major importance for estrogen-mediated effects on gene transcription in HPLF cells and estrogen influences the expression of OPG in HPLF cells via ERβ.

Key concepts: Osteoprotegerin, Periodontal fiber, Estrogen receptor, Western blot, Internal medicine, Small interfering RNA, Estrogen, Gene expression

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Effect of estrogen receptor beta on the expression of osteoprotegerin in human periodontal ligament cells — Research Paper | ScholarLens