2007•PubMedRequires access

[The expression of estrogen receptors in the human periodontal ligament fibroblasts in vitro].

Meng Cao, Yin Ding, Lei Shu, Jia Li, Wei Zhang, Tao Guo

Abstract

OBJECTIVE: To explore the expression of estrogen receptors in the human periodontal ligament fibroblasts in vitro. METHODS: Human periodontal ligament fibroblasts were cultured in vitro. The estrogen receptors (ER)-alpha, ER-beta were detected with immunocytochemistry staining; The mRNA of ER-alpha, ER-beta were measured by reverse transcription polymerase chain reaction; The expression of ER-alpha, ER-beta protein were detected by Western blot. RESULTS: The mRNA and protein expression of two subtype of ER were observed on human periodontal ligament fibroblasts (HPLF). The intensity of the ER-beta bands were stronger than those of ER-alpha, indicating that ER-beta expression in HPLF was higher. CONCLUSIONS: ER may play an important role in the function of human periodontal ligament fibroblasts.

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Key concepts: Periodontal fiber, Western blot, Estrogen receptor, In vitro, Receptor, Estrogen receptor beta, Estrogen receptor alpha, Immunocytochemistry