Construction and identification of human IL-17RC and IL-17RA -specific shRNA eukaryotic expression vector

Objective To construct eukaryotic expression vector of shRNA specific for human IL-17 receptor C (IL-17RC).Methods ccording to the published target sequence of IL-17RC, oligonucleotides were designed and synthesized, annealed and inserted into pGenesil-1 subsequently. The recombinant plasmid was confirmed by restriction enzyme digestion and DNA sequencing. The recombinant eukaryotic expression vector was transfected into 293T cells, IL-17RC mRNA levels were detected by flow cytometry.Results The construction of IL-17RC shRNA expression vector were confirmed successfully by the restriction digestion and sequencing. Flow cytometry showed that IL-17RC expression level with IL-17RC-shRNA positve cells was significantly lower than that of wild-type cells.Conclusions The construction of IL-17RC shRNA expression vector lays the foundation for the study of the function of IL-17 in osteoarthritis and the design of new drug for osteoarthritis treatment.