Construction of eukaryotic vector expressing shRNA of ovarian cancer associated antigen CD147

Objective To construct eukaryotic vector expressing shRNA (short hairpin RNA) of CD147. Methords Designing three different shRNA targeting the coding sequence of the CD147, the pGE-CD147 shRNA was constructed by inserting the designed shRNA to the eukaryotic expression vector pGE-1.The human ovarian cancer cell strain HO-8910pm was transfected by pGE-CD147 shRNA. After selected by G418,the CD147 expression in the transfected 8910pm cells was detected by RT-PCR and Western blot.Results It was verified by partial nucleotide sequencing and restriction endonuclease digestion that the constructed eukaryotic vector expressing shRNA of CD147 was correct.The CD147 expression was significantly suppressed in 8910pm cells transfected by pGE-CD147 shRNA as compared with untransfected 8910pm cells. Conclusion The results of the study lay the foundation for further studying on biological functions and potential application of CD147 and give some guidance for the shRNA design.