2002•Unpublished venueRequires access
Synthesis and identification of B-cell epitope of tumor antigen MAGE-12
Yan Li
Open publisher page 0 citations
Abstract
Objective To synthesize and identify the antigenic peptide of B cell epitope on tumor antigen MAGE 12. Methods Based on predicted B cell epitope MAGE 12 82-93 , a multiple antigenic peptide (MAP) system was used to synthesize the immunogen. C 57BL/6 mice were immunized with the immunogen to produce the polyclonal anti serum. The polyclonal antibody in the serum of immunized mice was identified by using ELISA and immunohistochemical method. Results The antibody titer was reached to 1∶64. The polyclonal antibody was identified as objective specific antibody. Conclusion Those results show that the synthesized MAGE-1282-93 may be a B-cell epitope of MAGE 12.