Comparative studies on lignin degradation enzymes produced by three species of white-rot fungi and combination of the strains

Trametes cinnabarina,Pleurotus ostreatus and Phanerochaete chrysosporium are high-yielding strains producing lignin degradation enzymes.Comparative studies on lignin degradation enzymes produced by these three species of white-rot fungi and combination of the strains were conducted.The results showed that liquid-shaking culture was the best culture method,yeast extract liquid medium was the best medium.As far as laccase(lacs) production was concerned,the enzyme activities of lacs produced by the combination of Pleurotus ostreatus and Phanerochaete chrysosporium were the highest,reaching 450U/L on day 6;the enzyme activities of manganese peroxidases(mnps) produced by the combination of Trametes cinnabarina and Pleurotus ostreatus were the highest,reaching 1050U/L on day 10;the enzyme activities of lignin peroxidases(lips) produced by the combination of Trametes cinnabarina and Phanerochaete chrysosporium were the highest,reaching 2990U/L on day 8.The activities of three kinds of main lignin degradation enzymes produced by combination of the three species of white-rot fungi were much higher than those produced by single strain of the fungi.It is suggested that combined strains might be effective for increasing the yield of enzyme production.