Study on Effects of Adenosine on Myocardium and Endothelin-1 during Ischemia-Reperfusion Injury in Rabbits

Objective To evaluate the effects of adenosine on myocardium and endothelin-1(ET-1) during ischemia-reperfusion injury in rabbits in order to explore the myocardioprotective effect and mechanism of pretreatment of adenosine during ischemia-reperfusion.Method Twenty-four healthy rabbits were randomly divided into 3 groups(8 rabbits each):control group,adenosine pretreatment group and adenosine treatment group.Myocardial ischemia-reperfusion models were made by means of ligating the left anterior descending branch of coronary artery for 40 minutes,followed by 3 hours of reperfusion.The level of creatine kinase(CK) and the weight of infarct myocardium were detected before ischemia and after 3 hours of reperfusion.The levels of superoxide dismutase(SOD),malonaldehyde(MDA) in each group were also compared.The plasma levels of ET-1 were measured with an immnoradiometric assay before ischemia,during reperfusion as well as 30,60,120 minutes after reperfusion.Results Compared with control group and adenosine treatment group,the levels of CK,SOD,MDA in adenosine pretreatment group significantly reduced(P0.01) after 3 hours of reperfusion,the infarct size significantly reduced(P0.01),the levels of ET-1 were significantly lower(P0.01) except before ischemia.Conclusions Adenosine pretreatment can reduce the ischemia-reperfusion injury.It has significant protective effects on ischemia-reperfusion myocardium.Adenosine may reduce the level of ET-1,protect endothelial cells and reduce the ischemia-reperfusion injury.