2012•Guide of China MedicineRequires access

Comparison of A Variety of Detection Methods for Syphilis

Huang JianCheng

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Abstract

Objective Application of a variety of different serological detection methods to determine syphilis antibodies of the serum samples and evaluation of test results.Methods 100 blood specimens collected syphilis patients,respectively,using enzyme-linked immunosorbent assay(ELISA),treponema pallidum gelatin particle agglutination(TPPA),colloidal gold rapid testing(ACONSYP),toluidine red unheated serum test(TRUST) serum syphilis.Results TP-ELISA screening test for syphilis-positive serum of 100 patients,several other methods are positive,94 TRUST positive and 94 of TPPA positive 98,the SYP positive 96.The control group of 120 non-syphilis by the number of TP-ELISA tested negative for 114 and a specificity of 95%.Conclusion There are a variety of methods of false negative and false positive for TP-ELISApositive samples,in order to improve syphilis detection rate,do TRUST and TPPA re-examination.

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Objective Application of a variety of different serological detection methods to determine syphilis antibodies of the serum samples and evaluation of test results.Methods 100 blood specimens collected syphilis patients,respectively,using enzyme-linked immunosorbent assay(ELISA),treponema pallidum gelatin particle agglutination(TPPA),colloidal gold rapid testing(ACONSYP),toluidine red unheated serum test(TRUST) serum syphilis.Results TP-ELISA screening test for syphilis-positive serum of 100 patients,several other methods are positive,94 TRUST positive and 94 of TPPA positive 98,the SYP positive 96.The control group of 120 non-syphilis by the number of TP-ELISA tested negative for 114 and a specificity of 95%.Conclusion There are a variety of methods of false negative and false positive for TP-ELISApositive samples,in order to improve syphilis detection rate,do TRUST and TPPA re-examination.

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Available abstract

Objective Application of a variety of different serological detection methods to determine syphilis antibodies of the serum samples and evaluation of test results.Methods 100 blood specimens collected syphilis patients,respectively,using enzyme-linked immunosorbent assay(ELISA),treponema pallidum gelatin particle agglutination(TPPA),colloidal gold rapid testing(ACONSYP),toluidine red unheated serum test(TRUST) serum syphilis.Results TP-ELISA screening test for syphilis-positive serum of 100 patients,several other methods are positive,94 TRUST positive and 94 of TPPA positive 98,the SYP positive 96.The control group of 120 non-syphilis by the number of TP-ELISA tested negative for 114 and a specificity of 95%.Conclusion There are a variety of methods of false negative and false positive for TP-ELISApositive samples,in order to improve syphilis detection rate,do TRUST and TPPA re-examination.

Key concepts: Medicine, Syphilis, Treponema, Serology, Gold standard (test), Antibody, Internal medicine, Immunology

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