Research on anarobic cultural condition of succinic acid producing strain Actinobacillus succinogenes 130Z

The investigation of fermentation conditions of Actinobacillus succinogenes 130Z was conducted.Effects of nitrogen sources,ratio of C to N,CO2 donor,temperature and broth initial pH on the content of succinic acid and biomass of A.succinogenes 130Z were studied.Results showed that the optimum medium was as follows(in g/L): glucose 10,yeast extract 5,NaHCO3 10,Na2HPO4 0.3,NaH2PO4·2H2O 9.6,K2HPO4 3,MgCl2 0.2,MnCl2 0.2,NaCl 0.1,pH 7.0.Under the optimal conditions,the concentration of succinic acid in serum bottle with cultivate temperature 37 ℃ and cultivation period 24h reached 8.3 g/L.The process was scaled up in a 5 L fermenter.When the initial concentration of glucose were 10 g/L and 100 g/L,the amount of succinic acid reached 8.2 g/L and 45.6 g/L with the yield of 80% and 65%,respectively.