Study on the Quality of Medicinal Material of Herba Taraxaci Produced in Shanxi

Objective: To determine the contents of caffeic acid and chlorogenic acid in herba taraxaci produced in Shanxi by using HPLC method. Methods: The octadecylsilane chemically bonded silica was taken as the filler, and the phosphoric acid(he phosphate solution containing 0.1% phosphoric acid): acetonitrile(85:15)as flowing phase with a flow rate of 1.0 ml/min and a detection wavelength of 322 nm. Results: The linear range of caffeic acid and chlorogenic anid were 0.8 μg/mL ～4.0 μg/mL(r=0.9966)and 0.4 μg/mL～2.0 μg/mL(r=0.9997)respectively; the average recovery of caffeic acid and chlorogenic acid was 98.73 % with RSD of 2.49% and 98.82 % with RSD of 2.71% respectively. Conclusion: The mass fraction of caffeic acid and chlorogenic acid was 0.074% and 0.028% respectively, the quality is good.