ScholarLens Back to search
2008•Shiyong yaowu yu linchuangRequires access

Determination of chlorogenic acid and caffeic acid in Herba taraxaci by RP-HPLC

Dan Li

Open publisher page 0 citations

Abstract

Objective RP-HPLC method was established to determine chlorogenic acid and caffeic acid in Herba taraxaci.Methods Diamonsil C18 column was used with a mixture of acetonitrile and sodium phosphate buffer(13∶87,v/v) as mobile phase.Results The linear ranges were 2.4～48.0 μg/mL(r=0.999 5),1.0～20.0 μg/mL(r=0.999 7) respectively.The recoveries were 99.7%(RSD=0.8%),100.8%(RSD=1.1%) respectively.Conclusion The assay method is simple,rapid and with good reproducibility and,it could provide the quality assessment for Herba taraxaci.

About this research paper

What this paper is about

Objective RP-HPLC method was established to determine chlorogenic acid and caffeic acid in Herba taraxaci.Methods Diamonsil C18 column was used with a mixture of acetonitrile and sodium phosphate buffer(13∶87,v/v) as mobile phase.Results The linear ranges were 2.4～48.0 μg/mL(r=0.999 5),1.0～20.0 μg/mL(r=0.999 7) respectively.The recoveries were 99.7%(RSD=0.8%),100.8%(RSD=1.1%) respectively.Conclusion The assay method is simple,rapid and with good reproducibility and,it could provide the quality assessment for Herba taraxaci.

Why it matters

A significance statement is not available in the OpenAlex record.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

Objective RP-HPLC method was established to determine chlorogenic acid and caffeic acid in Herba taraxaci.Methods Diamonsil C18 column was used with a mixture of acetonitrile and sodium phosphate buffer(13∶87,v/v) as mobile phase.Results The linear ranges were 2.4～48.0 μg/mL(r=0.999 5),1.0～20.0 μg/mL(r=0.999 7) respectively.The recoveries were 99.7%(RSD=0.8%),100.8%(RSD=1.1%) respectively.Conclusion The assay method is simple,rapid and with good reproducibility and,it could provide the quality assessment for Herba taraxaci.

Key concepts: Chlorogenic acid, Chromatography, Caffeic acid, Chemistry, High-performance liquid chromatography, Phosphate buffered saline, Biochemistry, Antioxidant

Related papers

Related paper 1Related paper 2Related paper 3
Back to paper searchBrowse research topicsOriginal source
Determination of chlorogenic acid and caffeic acid in Herba taraxaci by RP-HPLC — Research Paper | ScholarLens