Alteration of LXR Expression in Rats Lung With Acute Injury

Objective To investigate the expression and potential role of liver X receptors(LXRs)in lung of rats with acute lung injury induced by lipopolysaccharide(LPS).Methods 50 male SD rats were divided randomly into five groups:NS(normal saline)control group,LPS injured after 1 h group(LPS 1 h),LPS injured after 2 h group(LPS 2 h),LPS injured after 4 h group(LPS 4 h)and LPS injured after 8 h group(LPS 8 h).Semi-quantitative reverse transcription polymerase(RT-PCR) and western-blot were used to measure expression of LXRs.The ratio of lung wet-dry weight(W/D) and arterial blood gas analysis were measured respectively.Results Compared to the control group,the ratio of lung wet/dry weight was significantly increased in LPS injured groups,the PaO2 was significantly decreased.The expression of LXRα/β mRNA begins to decrease at 2 h after LPS injured and significantly decreased at 4h and 8h group,While the expression of LXRα/β protein begins to decrease at 4 h after LPS injured and significantly decreased at 8h group.Conclusion The expression of LXRs in acute lung injury(ALI) of rats induced by LPS significantly decreased.The data suggested that LXRs might involve in the regulation of inflammation in ALI.