Establishment and application of RT-LAMP technique in detection of enterovirus 71 gene

A simple and sensitive Reverse Transcription Loop-mediated Isothermal Amplification(RT-LAMP) method was established to provide a new rapid method for detecting Enterovirus 71 gene.A set of six specially primers that recognized eight distinct sequences of VP1 gene of EV71 were designed,RT-LAMP method was established,and the specificity and sensitivity of RT-LAMP were verified.The stool or throat specimens of patients infected with Hand Foot and Mouth Disease(HFMD) were tested by RT-LAMP technique,and the result was compared to that of RT-PCR and Real-time PCR.Results demonstrated that the RT-LAMP technique has been successfully detected VP1 gene of EV71,and showed a good consistency with Real-time PCR results but higher detection rates than RT-PCR method.It's suggested that RT-LAMP technique appeared to be high sensitivity and specificity,as well as simple and rapid.It is suitable for the diagnosis of HFMD and for molecular epidemiological surveillance of infection,and with good development prospects.