The Expression and Antibacterial Effect of Antimicrobial Peptides Spinigerin α' Gene

In this study,Pichia pastoris GS115 as expression host is for antimicrobial peptides Spinigerin α' gene extracellular secretion expression.Using agar hole diffusion method,on E.coli-K12D31,Staphylococcus aureus and Salmonella enteritidis as sensitive strains,for antimicrobial peptides Spinigerin α' expression liquid antibacterial.The results show that the antibacterial activity of the Spinigerin α'-1 reduces,Spinigerin α'-2 antibacterial activity increases slightly,Spinigerin α'-3 inhibition activity is strongest,and Spinigerin α'-4 and the 5 yeast expression protein have no antibacterial activity.The trial has laid an important foundation for research of antimicrobial peptides antibacterial effect improved.