Effects of Progesterone on TGF-β1 and TβR1 in Ishikawa Cell of Endometrial Carcinoma

Objective To investigate the effects of progesterone(MPA)on apoptosis and TGF-β1、TβR1 protein expression of Ishikawa cell line of human endometrial carcinoma in vitro.Methods The proliferation of Ishikawa cells in vitro was determined by methyl thiazolyl terazolium(MTT)method.Percentage of cell apoptosis was detected by flow cytometry(FCM).The expression of TGF-β1、TβR1 protein of Ishikawa cells was detected by streptavidin-peroxidase linkage(SP)method.Results Progesterone inhibited the growth of Ishikawa cells significantly when the concentration of progesterone reached 15 μg/ml(P 0.01).Flow cytometry(FCM)showed that the apoptotic rate in progesterone-treated group was significantly increased(P 0.05)compared with control group.Apoptosis morphology was observed in each group.The expression of TGF-β1 in Ishikawa cells was decreased after the cells had been treated with MPA for 72 hours,while expression of TβR1 was increased.Conclusion Progesterone inhibited the proliferation and induced the apoptosis of Ishikawa cells.The regulation on the expressions of the TGF-β1 and TβR1 proteins by progesterone seems to be responsible for the effects on the apoptosis of Ishikawa cells.