Effects of progesterone on Survivin in ishikawa endometrial carcinoma cell line

Objective To investigate the effects of progesterone on apoptosis and Survivin expression protein of Ishikawa cell line of human endometrial carcinoma in vitro.Methods The proliferation of Ishikawa cells in vitro was determined by methyl thiazolyl terazolium (MTT) medhod.Cell cycle,apoptotic percentage were detected by cytometer. The expression of Survivin protein of Ishikawa cells was detected by immunocytochemistry. Results Progesterone inhibited the growth of Ishikawa cells significantly when the concentration of progesterone reached at 0.5×10-5～10-4mol/L(P0.01). Flow cytometry(FCM) showed that the proportion percentage of G0/G1 phase and the apoptotic rate were significantly increased. The proportion of S phase was reduced(P0.01). Apoptosis morphology was observed in each group. The expression of Survivin by Ishikawa cells was decreased after the cells was treated with P. Conclusion Progesterone inhibited the proliferation and induced apoptosis of Ishikawa cells. The regulation on the expression of the Survivin protein by progesterone seems to be responsible for the effects on the apoptosis in Ishikawa cells.