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2012•Xi'nan nongye xuebaoRequires access

Indication of Genetic Linkage Map for Sunflower by SSR Markers

Huang XianQun

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Abstract

This study aimed to improve density and practicality of the genetic map of sunflower based on a 125 F8 RILs population derived from a cross between PAC-2 and RHA-266 by adding some SSR markers.A total of 300 pairs of SSR primers were used to screen polymorphic markers between the parents and some of their RILs,of which 51 pairs of the primers showed polymorphism.The results of screening the RILs population revealed that 19 SSR primer without polymorphism or non-reading,32 SSR pairs showed polymorphism with 35 alleles added into the map.They were distributed in the 15 linkage groups of the maps.The new map covered a total length of 2914.5 cM,7.5 cM longer than the original map.The average distance between adjacent markers was 8.1 cM instead of original 9.0 cM.

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What this paper is about

This study aimed to improve density and practicality of the genetic map of sunflower based on a 125 F8 RILs population derived from a cross between PAC-2 and RHA-266 by adding some SSR markers.A total of 300 pairs of SSR primers were used to screen polymorphic markers between the parents and some of their RILs,of which 51 pairs of the primers showed polymorphism.The results of screening the RILs population revealed that 19 SSR primer without polymorphism or non-reading,32 SSR pairs showed polymorphism with 35 alleles added into the map.They were distributed in the 15 linkage groups of the maps.The new map covered a total length of 2914.5 cM,7.5 cM longer than the original map.The average distance between adjacent markers was 8.1 cM instead of original 9.0 cM.

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Available abstract

This study aimed to improve density and practicality of the genetic map of sunflower based on a 125 F8 RILs population derived from a cross between PAC-2 and RHA-266 by adding some SSR markers.A total of 300 pairs of SSR primers were used to screen polymorphic markers between the parents and some of their RILs,of which 51 pairs of the primers showed polymorphism.The results of screening the RILs population revealed that 19 SSR primer without polymorphism or non-reading,32 SSR pairs showed polymorphism with 35 alleles added into the map.They were distributed in the 15 linkage groups of the maps.The new map covered a total length of 2914.5 cM,7.5 cM longer than the original map.The average distance between adjacent markers was 8.1 cM instead of original 9.0 cM.

Key concepts: Sunflower, Genetic linkage map, Biology, Genetics, Allele, Population, Genetic linkage, Genetic marker

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