Effect of Valsartan and Spironolacton on Growth Factors and TypeI Collagen in Left Ventricle in Spontaneously Hypertensive Rat

Objective To observe the effect of AT 1 receptor antagonist and mineralocorticoid receptor antagonist on growth factors and type Ⅰ collagen in left ventricle in spontaneously hypertensive rat(SHR) Methods Six week male SHRs( n =18) were randomly to received valsartan (30 mg·kg -1 ·d -1 ), spironolactone (20 mg·kg -1 ·d -1 ) or drinking water Wistar Kyoto rats(WKY)( n =6) were given normal water as control Expression of transforming growth factor β 1 (TGF β 1), hepatocyte growth factor(HGF) and type Ⅰ collagen gene mRNA in heart were determined by RT PCR Results Compared with those of untreated SHR group, cardiac levels of TGF β 1 and type Ⅰ collagen gene mRNA expression in valsartan and spironolactone groups were significantly reduced( P 0 01, respectively) at 13 wks, but remained higher than those in WKY group( P 0 01); cardiac levels of HGF mRNA expression in untreated SHR group were lower than those of WKY group( P 0 01) Compared with those of untreated SHR group, cardiac levels of HGF mRNA expression treated with valsartan and spironolactone were significantly increased( P 0 01, respectively), while still lower than those in WKY group Conclusion These results indicated that AngⅡ AT 1 receptor antagonist and mineralocorticoid receptor antagonist prevented myocardial fibrosis and left ventricular hypertrophy