2002Journal of Modern RehabilitationRequires access

The influence of Sanxiantang on the proliferation of osteoblast-like cell in vitro

Qin La

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Abstract

Objective By studying the influence of Sanxiantang on the proliferation of osteoblast like cells in vitro,to determine if there's a probability of using it in the treatment of menopausal osteoporosis.Methods Culturing the cell strain UMR106 in vitro,using NaF as the control medicine, we observed the effects of Sanxiantang on the proliferation of osteoblast like cells by applying the MTT assay.Results NaF could significantly promoted the proliferation of osteoblast like cells at the concentration of 1.25 μmol/L(P 0.01),5 μmol/L (P 0.001), having no significant difference at 10 μmol/L compared with control.While the Sanciantang at the concentrations of 5,10,100,500,1 000 mg/L all could significantly promoted the proliferation of osteoblast like cells in vitro(P 0.001,P 0.01).Conclusions Sanxiantang could significantly promote the proliferation of osteoblast like cells in vitro.

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Objective By studying the influence of Sanxiantang on the proliferation of osteoblast like cells in vitro,to determine if there's a probability of using it in the treatment of menopausal osteoporosis.Methods Culturing the cell strain UMR106 in vitro,using NaF as the control medicine, we observed the effects of Sanxiantang on the proliferation of osteoblast like cells by applying the MTT assay.Results NaF could significantly promoted the proliferation of osteoblast like cells at the concentration of 1.25 μmol/L(P 0.01),5 μmol/L (P 0.001), having no significant difference at 10 μmol/L compared with control.While the Sanciantang at the concentrations of 5,10,100,500,1 000 mg/L all could significantly promoted the proliferation of osteoblast like cells in vitro(P 0.001,P 0.01).Conclusions Sanxiantang could significantly promote the proliferation of osteoblast like cells in vitro.

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Available abstract

Objective By studying the influence of Sanxiantang on the proliferation of osteoblast like cells in vitro,to determine if there's a probability of using it in the treatment of menopausal osteoporosis.Methods Culturing the cell strain UMR106 in vitro,using NaF as the control medicine, we observed the effects of Sanxiantang on the proliferation of osteoblast like cells by applying the MTT assay.Results NaF could significantly promoted the proliferation of osteoblast like cells at the concentration of 1.25 μmol/L(P 0.01),5 μmol/L (P 0.001), having no significant difference at 10 μmol/L compared with control.While the Sanciantang at the concentrations of 5,10,100,500,1 000 mg/L all could significantly promoted the proliferation of osteoblast like cells in vitro(P 0.001,P 0.01).Conclusions Sanxiantang could significantly promote the proliferation of osteoblast like cells in vitro.

Key concepts: Osteoblast, In vitro, Cell growth, Chemistry, MTT assay, Cell, Andrology, Cell biology

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