Effect of Increasing CO_2 Concentration on Rice Leaf Microstructure

In order to determine the effect of increasing CO2concentration on rice leaf microstructure, open-top chamber was used for exposing a japonica rice variety in two CO2concentrations 380 μmol·mol-1(CK) and 550 μmol·mol-1(Treatment).The fourth leaf epidermal cells of rice were examined in the scanning electron microscopy.The results indicated that the higher CO2concentration changed epidermal cells distribution.The effects were an significantly increase in space between silicon bolt and stomatal band(16.41%,22.11%),Glandular hairs(79.04%) of the leaf on the back and front, and decreased in stomatal index(14.18%,14.80%),space between stomata(20.28%,14.73%) of the leaf on the back and front,back trichome(48.87%).Changes in the distribution of the leaf on the back of the cell was less than the ventral, and the back leaf stomatal index decrease was smaller than the ventral surface, making differences between the back and the ventral much bigger.The increasing CO2concentration made quite difference in stomatal character between back leaf and front leaf side.The higher CO2concentration result in significantly change in stomatal area, stomatal perimeter, stomatal length of the back side increased by 19.07%,17.56%,21.17%,respectively,and leaf front stomatal width, papilla of back and front leaf decreased by 9.18%,17.34%,9.50%.The stomatal character of back leaf change much bigger than front leaf.