Effects of Stromal Cell-Derived-Factor-1 on Endothelial Progenitor Cells of Peripheral Blood and Their Relationship with PI3K/AKT Signal Transduction Pathway in Patients with Diabetes

Objective To observe the effects of stromal cell-derived-factor-1(SDF-1) on the function of endotheli-al progenitor cells(EPCs)of peripheral blood in patients with diabetes, and to discuss the effects of PI3K/AKT signaling path-way on the role of SDF-1 in EPCs. Methods The peripheral blood samples(30 m L) were collected in 10 diabetes patients(DM group) and 10 healthy controls(HC group).(1) The 100 μg/L SDF-1 was added in intervention group. EGM-2MV wasadded in non-intervention group. The Boyden chamber and in vitro angiogenesis kit were used to analyze the migration andin vitro angiogenesis of EPCs.(2) Cultured EPCs were divided into blank control group, 1 μg/L SDF-1 group, 10 μg/L SDF-1 group, 100 μg/L SDF-1 group, pure AMD3100 group and 100 μg/L SDF-1+AMD3100 group. AKT protein expression lev-els of endothelial progenitor cells were detected by Western blot assay in each group. Results(1) Without intervention withSDF-1, EPCs' migration and angiogenesis ability were lower in DM group than those in HC group. After intervention withSDF-1, the migration and angiogenesis ability were enhanced in two groups, but the increased level was higher in DM groupthan that of HC group.(2) Under the same concentration, AKT protein expression level was significantly lower in DM groupthan that in HC group(P 0.01). AKT protein expression levels were increased with the increased levels of SDF-1 in DMgroup and HC group(P 0.05). AKT protein expression was significantly lower in 100 μg/L SDF-1+AMD3100 group thanthat of 100 μg/L SDF-1 group(P 0.05). Conclusion SDF-1 can increase the chemotactic migration and angiogenesisability of EPCs in peripheral blood, especially for patients with diabetes. The effects of SDF-1 on EPCs were related to thePI3K/AKT signaling pathway.