Effects of SDF-1α on biological function of EPCs in the patients with glucose metabolism disorders

Objective To clarify the effect of stromal cell-derived factor-1α(SDF-1α) on the biological function of circulating endothelial progenitor cells(EPCs) in the patients with glucose metabolism disorders.Methods The peripheral blood was collected from the 60 normal adults,78 patients with impaired glucose tolerance,71 T2DM patients without vascular complications,and 60 T2DM patients with vascular complications.The isolation of mononuclear cells was by density gradient centrifugation,followed by identification of EPCs.The amount,migration,adhesion,proliferation,and in vitro angiogenesis of EPCs were measured respectively.SDF-1α was determined by enzyme-linked immunosorbent assay(ELISA).The differences in SDF-1 and EPCs between different groups were analyzed.The correlation of SDF-1α with EPCs′ amount,migration,adhesion,proliferation,and angiogenesis in vitro were observed.Results In the normal control group,SDF-1α was 6.52±1.04 μg/L;in the IGT group,it was 6.23±1.02 μg/L;in the T2DM without vascular complications group,it was 5.98±0.97 μg/L;and in the T2DM with vascular complications group,it was 5.39±0.85 μg/L.EPCs′ amount,migration,adhesion,proliferation,and angiogenesis in vitro were 53.7±6.7 cell/200,35.17±4.39 cell/200,25.98±4.39 cell/200,17.24±2.15 cell/200,9.7±2.2 cell/200,6.27±1.42 cell/200,4.11±0.93 cell/200,2.35±0.53 cell/200,22.9±4.3 cell/200,17.36±3.26 cell/200,12.36±2.32 cell/200,7.73±1.43 cell/200,0.62±0.04 cell/200,0.51±0.03 cell/200,0.38±0.03 cell/200,0.29±0.02 cell/200,11.3±1.8 tubules/200,8.82±1.32 tubules/200,5.69±0.91 tubules/200,and 4.10±0.65 tubules/200(P0.01).The correlation analysis showed that SDF-1α was positively correlated only with the amount of EPCs in the IGT group(P0.05),while it was correlated positively with the amount,migration,adhesion,proliferation,and angiogenesis in vitro in the T2DM with and without vascular complications groups(P0.05 or P0.01).Conclusion In the patients with glucose metabolism disorders,SDF-1α impairs EPCs.This finding provides a new theoretical basis for making strategy in treatment of T2DM.