Appraisal of mechanism of preventing intimal hyperplasia on vascular anastomotic site by locally delivered antisense c-myc oligodeoxynucleotide

Objective To elucidate the mechanism of attenuation of vascular intimal hyperplasia by antisense c myc oligodeoxynucleotide.Methods Eight male New Zealand white rabbits with external jugular veins interposed between ipsilateral common carotid arteries were randomly and equally divided into two groups. The antisense c myc oligomer of 330 μg was dissolved in fibrin gel and applied to the outer layer of vascular anastomotic site in the experimental group. Seven days later, the animals were sacrificed and the total RNA of the specimens was extracted. RT PCR and Northern blot analysis were carried out and the pictures were scanned and digitalized by computer.Results Both RT PCR and Northern blot analysis revealed a significant reduction of c myc expression in antisense c myc oligodeoxynucleotide treated tissues.Conclusion The results implied that the antisense c-myc oligomers can bind to the target mRNAs in accordance with the base complementary principle after entering the tissue cells. The target gene expression was inhibited and the cell proliferation was suppressed with eventual prevention of restemosis induced by intimal hyperplasia.