Effect of Xiaoguruanjian Pill on Blood TNF,IL-1,IL-6,NO and ET-1 of Rats with Liver Cirrhosis

To study the effect of Xiaoguruanjian pill(XP) on blood TNF、IL-1, IL-6, NO and ET-1 of rats with liver cirrhosis. Methods:Rat models of liver Cirrhosis were made with multiple factors (mainly by injecting CCl_4). Sixty Wistar rats were randomly divided into five groups: normal control group, model control group, low dose XP group, high dose XP group and positive control group(Biejiaruangan Tablet). From the second day after the inducement of the models, all rats were continuously infused with saline or the medicine for 28 days, and were cut off head for obtaining blood after the last medication. The blood was centrifuged and separated for serum and blood plasma in tow temperature. The contents of TNF, IL-1, IL-6, NO and ET-1 were detected separately. Results: 1. After the medication, the contents of serum IL-1, IL-6, TNF in each treatment group were lower than those of model group (P0.01), the contents of serum IL-1, IL-6, TNF in high-dose XP group were lower than those of control group( P0.05 or P0.01),the contents of serum IL-1 and TNF in low-dose XP group were lower than those of control group(P0.01), and there was no significant difference between high-dose group and low-dose group(P0.05). 2. After the medication, the contents of serum NO and NOS in each treatment group were lower than those of model group (P0.01), the contents of serum NO and NOS in high-dose XP group were lower than those of low-dose XP group and control group(P0.01),the content of serum NOS in low-dose XP group was lower than that of control group(P0. 01). 3. After the medication, the content of plasma ET-1 in each treatment group was lower than that of model group (P0.01), the content of plasma ET-1 in high-dose group was lower than that of low-dose XP group and control group(P0.01). Conclusion. 1. XP can decrease obviously the level of serum TNF, IL-1, IL-6,which abnormally are heightened in rat models of liver cirrhosis. 2. XP can lower the contents of serum NO, NOS of and plasma ET-1, which indicates that this prescription can alleviate inflammation injury, improve system hyperkinetic circulatory state, relieve portal hypertension and liver microcirculation disorder, decrease or restrain the happening or development of ascites.