Observation of Zhimaining on leptin and TNF-α in rat serum with alcoholic fatty liver

Objective To observe the effect of Zhimaining on leptin and TNF-α in rat serum with alcoholic fatty liver(AFL),and discuss possible mechanism of Zhimaining on AFL.Methods 70 Wistar rats(weight from 160~180 g) were randomly divided into five groups,control group,model group,high dose Zhimaining group,low dose Zhimaining group and Dongbaogantai group.AFL model was established by feeding high fat diet and intragastric administration of white spirit.There were 15 rats in each group except 10 rats in control group.Control group was given distilled water,eating normal diet,free access to water.Low dose Zhimaining group and high dose Zhimaining group were respectively fed Zhimaining 0.75,1.5 g/kg body weight.Dongbaogantai group was fed Dongbaogantai 0.9 g/kg body weight.The control group and model group were fed with distilled water.blood and liver tissue was collected under anesthesia.TC,TG,AST,ALT,Leptin and TNF-α in serum and pathological changes in liver tissue were observed after eight weeks.Results TC,TG,ALT and AST in high dose Zhimaining group,low dose Zhimaining group and Dongbaogantai group were significantly decreased in comparison with those in model group(P0.01).TC,TG,ALT and AST in high dose Zhimaining group were lower than those in Dongbaogantai group(P0.05,P0.01).TC in low dose Zhimaining group were lower than that in Dongbaogantai group(P0.05).The levels of leptin and TNF-α in model group were the highest in all groups(P0.01).The levels of leptin and TNF-α in high dose Zhimaining group and low dose Zhimaining group were lower than those in Dongbaogantai group(P0.05,P0.01).There was no difference between high dose Zhimaining group and low dose Zhimaining group on the levels of leptin and TNF-α(P0.05).Conclusion Zhimaining that reduces the levels of Leptin and TNF-α in serum may be one of the mechanism of prevention and treatment of AFL.