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Optimization of RAPD Reaction System in Cucumis melo

Zhang Qin-ying

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Abstract

Modified CTAB was used to extract genomic DNA from Cucumis melo L.Each factor in RAPD reaction system was studied.The optimal PCR system for RAPD analysis was as follows: the genomic DNA concentration was 2.5ng/ul,dNTP concentration was 0.25mmol/L,primer concentration was 0.80umol/L as well as Taq DNA polymerase concentration was 1U in 20ul reaction system.Based on this optimal PCR system,a dominant marker,550bp,was found using bulked segregation analysis strategy(BSA).

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What this paper is about

Modified CTAB was used to extract genomic DNA from Cucumis melo L.Each factor in RAPD reaction system was studied.The optimal PCR system for RAPD analysis was as follows: the genomic DNA concentration was 2.5ng/ul,dNTP concentration was 0.25mmol/L,primer concentration was 0.80umol/L as well as Taq DNA polymerase concentration was 1U in 20ul reaction system.Based on this optimal PCR system,a dominant marker,550bp,was found using bulked segregation analysis strategy(BSA).

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Available abstract

Modified CTAB was used to extract genomic DNA from Cucumis melo L.Each factor in RAPD reaction system was studied.The optimal PCR system for RAPD analysis was as follows: the genomic DNA concentration was 2.5ng/ul,dNTP concentration was 0.25mmol/L,primer concentration was 0.80umol/L as well as Taq DNA polymerase concentration was 1U in 20ul reaction system.Based on this optimal PCR system,a dominant marker,550bp,was found using bulked segregation analysis strategy(BSA).

Key concepts: RAPD, Cucumis, Primer (cosmetics), genomic DNA, Biology, DNA, Polymerase chain reaction, Chemistry

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