2000•Journal of Analytical ScienceRequires access

Determination of Trace Copper(II) by Catalytic Kinetic Spectrophotometric Method with Anlanine as the Sensitizer

Wang Xiao

Abstract

A kinetic spectrophotometric method for the determination of trace copper (Ⅱ) was established. It was based on reduction of neutral red (NR) with hydrogen peroxide (H 2O 2) in NH 3 NH 4Cl medium (pH=8.1) by means of Cu(Ⅱ) as catalyst and alanine as a sensitizer. The optimum reaction conditions and some kinetic parameters were studied. The determination range of copper was 0~3.2 μg/25 mL and the detection limit was 8.2×10 -11 g/mL. This method has been applied to the determination of Cu(Ⅱ) in biological samples with satisfactory results.

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Key concepts: Chemistry, Copper, Detection limit, Hydrogen peroxide, Catalysis, Kinetic energy, Trace Amounts, Spectrophotometry