Study on kanamycin and cefotaxime sensitivity of Eucalyptus grandis clone Eg5

By taking Eucalyptus grandis colne Eg5 leaves as explants,the effects of Kanamycin sulfate on the callus proliferation,bud differentiation and shoot rooting of leaf explants of Eucalyptus grandis clone Eg5 and the effect of Cefotaxime on explant's regeneration and the growth of Agrobacterium tumefaciens GV3101 were studied.The results showed that the concentration of kanamycin for restraining callus proliferation was 17.5 mg/L,that for fully suppressing bud differentiation was 60 mg/L and that for inhibiting shoots rooting was 400 mg/L.Cefotaxime at a dosage from 0 mg/L to 300 mg/L had minimal impact on the differentiation of leaf explants.With a soak of the explants in the solution of 500 mg/L Cefotaxime,the growth of agrobacterium can be fully inhibited at a Cefotaxime dosage of 100 mg/L in medium.