Effects of three antibiotics on callus induction and plant regeneration of Eucalyptus urophylla×Eucalyptus grandis

【Objective】 The study was to discuss the effects of hygromycin,kanamycin,and cephalosporin on the callus induction,regeneration of adventitious buds and root induction of Eucalyptus urophylla×Eucalyptus grandis.The optimum mass concentrations of cephalosporin to inhibition Agrobacterium was determined.【Method】 The asepsis stem segments of E.urophylla×E.grandis sterile clones were cultured respectively on the callus induction culture media,adventitious buds induction culture media,and rooting culture media,which were added several kinds of mass concentration antibiotics.Callus induction rate,rate of adventitious bud induction and rooting of adventitious buds were measured,respectively,and the effect of cephalosporin on Agrobacterium was observed.【Result】(1) The rate of callus induction from the explants was 6.7%,12.1% and 26.7% respectively,while the mass concentration of hygromycin,kanamycin and cephalosporin in the medium was higher than 15,25 and 200 mg/L.(2) Regeneration of adventitious buds was remarkably inhibited and the rate of regeneration of adventitious buds was 2.6%,10.2% and 7.6%,respectively while the mass concentrations of hygromycin,kanamycin and cephalosporin were over 15,25 and 250 mg/L.(3)Mullberry shoots could not form healthy roots and the rate of rooting of adventitious buds was 10.2%,16.2% and 10.1%,respectively while the mass concentrations of hygromycin,kanamycin and cephalosporin were 15,25 and 250 mg/L.Cefotaxime strongly inhibited Agrobacterium while the mass concentration of cephalosporin was over 200 mg/L.【Conclusion】 The inhibitive effects upon E.urophylla×E.grandis vary with the different antibiotics.The optimum mass concentrations of hygromycin and kanamycin were 10,20 mg/L at the stage of screening resistant callus and adventitious buds,and were 15,25 mg/L at the stage of rooting of resistant buds.200 mg/L Cef was a better choice to inhibit Agrobacterium.