Experimental study on protective effect of anisodamine on lung ischemia-reperfusion injury in rabbits

Objective To explore the protective effects of anisodamine(654-2) on lung and its possible mechanism during lung ischemia-reperfusion injury.Methods Single lung in situ warm ischemia reperfusion rabbit model was set up. Thirty Japanese rabbits were randomly divided into three equal groups, the control group, the IR group,and the ischemia-reperfusion group with anisodamine (8mg/kg) intravenous injection. The changes of lung tissue wet weight/dry weight ratio(W/D), malondialdehyda(MDA) and myeloperoxidase(MPO)activity, as well as the lung permeability index(LPI) were checked in each group. The histological appearances of the lungs were observed under histology and electron microscopy. Some sections were examined as an index of quantitative assessment of the degree of lung tissue damage(LTD).Results After 90 min ischemia and 120 min reperfusion, W/D,MDA, LPI, MPO activity, and LTD degree were significantly higher in IR group than those of the control group (P0.01).Anisodamine was effective in reducing W/D, MDA, LPI,MPO activity and LTD(P0.01). Histological examination of lung tissues demonstrated that the degree of lung injury had been alleviated significantly.Conclusion The lung tissue ischemia-reperfusion injury can be significantly protected with anisodamine. The protective mechanism may be that anisodamine can effectively reduce the neutrophil sequestration within the lung and the lung injury resulting from oxygen-derived free radical.