Isolation and identify of the rice OsGSTLc promoter

The expression of OsGSTLcin rice roots was induced by chlorsulfuron through semi-quantitative RT-PCR analysis demonstrated.A 2 171bp upstream sequence of the translation start codon ATG of OsGSTLc gene was isolated from the genomic DNA of rice,which contained a putative CAAT-box at position-86bp upstream of ATG,but there wasn't TATA-box between the CAAT-box and ATG.Therefore,OsGSTLc promoter was a TATA-less promoter.To define the core promoter sequence,a series of 5′truncation derivatives of GST2171were fused to a GUS reporter gene to construct GSTL2171::GUS,GSTL1761::GUS,GSTL962::GUSand GSTL525::GUS,respectively.The four fusion genes were introduced into rice plants by Agrobacterium-mediated transformation,all promoter fragments with 5′-deletion drived successfully the expression of GUS report gene.Quantitative fluorescence assays showed that the GUS activities in the roots of GSTL2171::GUS,GSTL1761::GUSand GSTL962::GUStransgenic rice seedlings were upregulated by chlorsulfuron.The transcriptional activation element of chlorsulfuron may be located between positions-962and-525.