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Isolation and Characterization of an Embryo-specific Promoter OsESP1 from Rice

Fang Xiao

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Abstract

A pair of specific primers were designed according to the 5′ upstream regulatory sequence of rice gene OsESG1, and a 1.4 kb fragement named OsESP1 was amplified using rice (Oryza sativa L. cv. Zhonghua 11) genomic DNA as template by PCR. OsESP1 was further fused with GUS reporter gene and transformed into rice callus through Agrobacterium-mediated transformation. GUS activities in various tissues of transformed plants were examined and the GUS activity was detected only in rice embryo, indicating the OsESP1 is an embryo-specific promoter.

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What this paper is about

A pair of specific primers were designed according to the 5′ upstream regulatory sequence of rice gene OsESG1, and a 1.4 kb fragement named OsESP1 was amplified using rice (Oryza sativa L. cv. Zhonghua 11) genomic DNA as template by PCR. OsESP1 was further fused with GUS reporter gene and transformed into rice callus through Agrobacterium-mediated transformation. GUS activities in various tissues of transformed plants were examined and the GUS activity was detected only in rice embryo, indicating the OsESP1 is an embryo-specific promoter.

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Available abstract

A pair of specific primers were designed according to the 5′ upstream regulatory sequence of rice gene OsESG1, and a 1.4 kb fragement named OsESP1 was amplified using rice (Oryza sativa L. cv. Zhonghua 11) genomic DNA as template by PCR. OsESP1 was further fused with GUS reporter gene and transformed into rice callus through Agrobacterium-mediated transformation. GUS activities in various tissues of transformed plants were examined and the GUS activity was detected only in rice embryo, indicating the OsESP1 is an embryo-specific promoter.

Key concepts: Oryza sativa, Biology, GUS reporter system, Transformation (genetics), Callus, Gene, Embryo, Agrobacterium

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Isolation and Characterization of an Embryo-specific Promoter OsESP1 from Rice — Research Paper | ScholarLens