Extraction processes and quality analysis of root bark and stem bark of Magnolia officinalis Rehd. et Wils.

Objective To study the optimum extraction processes of root bark and stem bark of Magnolia officinalis,with magnolol and honokiol as the assessment index.Methods Three methods,which included methanol merceration,ethanol refluxing extract and CO2 supercritical fluid extraction,were applied to extract magnolol,honokiol from Magnolia officinalis.The contents of magnolol and honokiol by the three extraction methods were assayed by HPLC.Results The contents of magnolol,honokiol were the highest by CO2 supercritical fluid extraction(2.00%,2.79% respectively).Conclusions Root bark and stem bark of Magnolia officinalis can be effectively extracted by CO2 supercritical fluid extraction;the HPLC analysis is convenient,easy and suitable to assay the contents of magnolol and honokiol from Magnolia officinalis.