Modulation by Yiqi Huoxue Recipe to the expression of TGF-β1 and the synthesis of procollagen typeI,III in radiation induced damage of lung of rats

Objective: To observe the modulation by Yiqi Huoxue Recipe to the expression of transforming growth factor beta(TGF-β1) and the synthesis of collagen type Ⅰ,Ⅲ in radiation-induced lung injury of rats in different radiation time,and discuss the action mechanism of prevention and curative effect of Yiqi Huoxue Recipe.Methods: Total of 96 wistar rats were randomly allocated into irradiation group,irradiation with Yiqi Huoxue Recipe-treated group,irradiation with ambroxol-treated group and normal control group.The first three groups were irradiated at right hemithorax with a dose of 30Gy/6 fraction/5weeks.Animals were followed for 26 weeks after the first irradiation.We evaluated the deposition of collagens and the level of tissue TGF-β1 by immunoreactivity.Results: The pulmonary fibrosis was remarkable at the late phase in irradiation group.The fibrosis of treatment group were lighter than irradiation group.The collagen fibers increased from the fourth week after the first radiation and the proportion of type I and Ⅲ collagen of irradiation group was significantly higher than the treatment group(P0.05) and the control group(P 0.05).Conclusion: Using Yiqi Huoxue Recipe earlier can reduce the synthesis of collagens effectively and inhibit radiation-reduced lung injury by restraining the expression of TGF-β1.