STUDY ON PREPARATION OF ANTIOXIDANT PEPTIDE FROM RICE BRAN USING NEUTRASE

The bioactive peptide solution with antioxidant ability was prepared from the hydrolysis of rice bran protein in water phase by use of neutral protease.The effects of the reaction temperature,time,dosage of enzyme(based on rice bran),pH and concentration of substrate(rice bran/water,g/mL) on the eliminating rate of free radicals OH· and O-2· of the bioactive peptide solution were investigated.The optimal reaction conditions are temperature 45 ℃,time 4.5 h,the dosage of enzyme 0.8%,pH 6.5~7.0,the concentration of substrate 1∶15.At the optimal reaction conditions the testing results indicated that the eliminating rate of OH· and O-2·of the bioactive peptide solution were 49.8% and 56.8% respectively.